Mouse PGF2α (Prostaglandin F2 Alpha) ELISA Kit

Mouse PGF2α (Prostaglandin F2 Alpha) ELISA Kit

Size

96 Tests

Catalog no.

EKE61847

Price

759 EUR

Buy

Research Area

NA

Stability

NA

Precision

NA

Specificity

NA

Target Name

NA

Species Reactivity

Mouse

Target's alterntive name

PGF2α

Assay Time

2.5 hours

Shipping Conditions

Ice packs

Sensitivity

9.38 pg/mL

Assay Type

Competitive

Latin name

Mus musculus

Detection Method

Colorimetric

Detection Range

15.63-1000 pg/mL

Estimated Turnaround Time

6-11 business days

Sample Type

Serum, Plasma, Biological Fluids

Storage Temperature

Short term: 4°C; Long term: see manual.

Application

For research use only. Not for diagnostic procedures.

Quality Systems

The kit is manufactured at ISO 9001 certified facilities.

Cross Activity

No significant cross-reactivity or interference was observed.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Shelf life

Use Mouse PGF2α (Prostaglandin F2 Alpha) ELISA Kit before 6 months

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Description

The Mouse PGF2α (Prostaglandin F2 Alpha) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Test Principle

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Mouse PGF2α. During the reaction, Mouse PGF2α in the sample or standard competes with a fixed amount of Mouse PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse PGF2α. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  Mouse PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.