Rat TM (Thrombin) ELISA Kit
Target's alterntive name
TM
Precision
NA
Stability
NA
Specificity
NA
Target Name
NA
Species Reactivity
Rat
Assay Type
Sandwich
Sensitivity
0.1 ng/mL
Assay Time
4.5 hours
Shipping Conditions
Ice packs
Detection Method
Colorimetric
Detection Range
0.16-10 ng/mL
Latin name
Rattus norvegicus
Estimated Turnaround Time
6-11 business days
Sample Type
Serum, Plasma, Biological Fluids
Research Area
Cancer, Cardiovascular, Stem cells
Storage Temperature
Short term: 4°C; Long term: see manual.
Shelf life
Use Rat TM (Thrombin) ELISA Kit before 6 months
Application
For research use only. Not for diagnostic procedures.
Quality Systems
The kit is manufactured at ISO 9001 certified facilities.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Cross Activity
No significant cross-reactivity or interference was observed.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test Principle
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TM . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TM and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TM. The concentration of Rat TM in samples can be calculated by comparing the OD of the samples to the standard curve.