Rat PGF2α (Prostaglandin F2 Alpha) ELISA Kit
Research Area
NA
Precision
NA
Stability
NA
Specificity
NA
Target Name
NA
Species Reactivity
Rat
Target's alterntive name
PGF2α
Assay Time
2.5 hours
Shipping Conditions
Ice packs
Sensitivity
4.69 pg/mL
Assay Type
Competitive
Detection Method
Colorimetric
Detection Range
7.81-500 pg/mL
Latin name
Rattus norvegicus
Estimated Turnaround Time
6-11 business days
Sample Type
Serum, Plasma, Biological Fluids
Storage Temperature
Short term: 4°C; Long term: see manual.
Application
For research use only. Not for diagnostic procedures.
Quality Systems
The kit is manufactured at ISO 9001 certified facilities.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Cross Activity
No significant cross-reactivity or interference was observed.
Shelf life
Use Rat PGF2α (Prostaglandin F2 Alpha) ELISA Kit before 6 months
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Description
The PGF2α (Prostaglandin F2 Alpha) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test Principle
This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat PGF2α. During the reaction, Rat PGF2α in the sample or standard competes with a fixed amount of Rat PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat PGF2α. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.