Rat PGF2α (Prostaglandin F2 Alpha) ELISA Kit

Rat PGF2α (Prostaglandin F2 Alpha) ELISA Kit

Size

96 Tests

Catalog no.

EKE62251

Price

759 EUR

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Research Area

NA

Precision

NA

Stability

NA

Specificity

NA

Target Name

NA

Species Reactivity

Rat

Target's alterntive name

PGF2α

Assay Time

2.5 hours

Shipping Conditions

Ice packs

Sensitivity

4.69 pg/mL

Assay Type

Competitive

Detection Method

Colorimetric

Detection Range

7.81-500 pg/mL

Latin name

Rattus norvegicus

Estimated Turnaround Time

6-11 business days

Sample Type

Serum, Plasma, Biological Fluids

Storage Temperature

Short term: 4°C; Long term: see manual.

Application

For research use only. Not for diagnostic procedures.

Quality Systems

The kit is manufactured at ISO 9001 certified facilities.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Cross Activity

No significant cross-reactivity or interference was observed.

Shelf life

Use Rat PGF2α (Prostaglandin F2 Alpha) ELISA Kit before 6 months

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Description

The PGF2α (Prostaglandin F2 Alpha) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test Principle

This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat PGF2α. During the reaction, Rat PGF2α in the sample or standard competes with a fixed amount of Rat PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat PGF2α. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  Rat PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.