Rabbit TAT (Thrombin/Antithrombin Complex) ELISA Kit
Precision
NA
Stability
NA
Specificity
NA
Target Name
NA
Target's alterntive name
TAT
Species Reactivity
Rabbit
Assay Type
Sandwich
Assay Time
4.5 hours
Shipping Conditions
Ice packs
Sensitivity
18.75 pg/mL
Detection Method
Colorimetric
Detection Range
31.25-2000 pg/mL
Estimated Turnaround Time
6-11 business days
Latin name
Oryctolagus cuniculus
Sample Type
Serum, Plasma, Biological Fluids
Storage Temperature
Short term: 4°C; Long term: see manual.
Application
For research use only. Not for diagnostic procedures.
Quality Systems
The kit is manufactured at ISO 9001 certified facilities.
Research Area
Cancer, Cell Biology, Cardiovascular, Signal transduction
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Cross Activity
No significant cross-reactivity or interference was observed.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Shelf life
Use Rabbit TAT (Thrombin/Antithrombin Complex) ELISA Kit before 6 months
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
About
Rabbits are used for polyclonal antibody production by Biomatik. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biomatik adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Test Principle
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit TAT . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit TAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit TAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit TAT. The concentration of Rabbit TAT in samples can be calculated by comparing the OD of the samples to the standard curve.