Coagulation Factor II (F2) Polyclonal Antibody (Mouse), Cy3
French translation
anticorps
Latin name
Mus musculus
Notes
Research Use Only.
Conjugation
cy3 conjugation kit
Group
Polyclonals and antibodies
Description
A Rabbit polyclonal antibody against Mouse Coagulation Factor II (F2). This antibody is labeled with Cy3.
Storage_and_shipping
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
Additional_information
Sequence of the immunogen: coagulation factor II (Ala44~Arg200); Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Specifications
Host: Rabbit; Species Reactivity: Mouse; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: Cy3
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Additional description
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Properties
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Coagulation Factor II (F2) Polyclonal Antibody (Mouse) can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Cloud Clone Corp antibody is well suited for fluorescent microscopy.