Recombinant Mouse Coagulation Factor II / FII / F2 Protein (His tag) 20µg

Recombinant Mouse Coagulation Factor II / FII / F2 Protein (His tag) 20µg

Size

20µg

Catalog no.

PKSM040723-20µg

Price

626 EUR

Buy

Fusion tag

C-His

AP Mol Mass

85 kDa

Mol Mass

69.3 kDa

Conjugation

histidine

Accession

NP_034298.1

Expressed Host

HEK293 Cells

Synonym

Cf-2;Cf2;FII

Group

recombinants

Latin name

Mus musculus

Sequence

Met 1-Gly 618

Source

Recombinants or rec. proteins

Purity

> 96 % as determined by SDS-PAGE

Formulation

Lyophilized from sterile PBS, pH 7.4

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Stability and Storage

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Activity

Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC R&D Systems, Catalog # ES011.The specific activity is > 2000 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Description

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Background

Coagulation Factor II Protein (FII, F2 Protein or Prothrombin) is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. Coagulation Factor II Protein (FII, F2 Protein) also plays a role in maintaining vascular integrity during development and postnatal life. Prothrombin / Coagulation Factor II is activated on the surface of a phospholipid membrane that binds the amino end of prothrombin / Coagulation Factor II and factor Va and Xa in Ca-dependent interactions; factor Xa removes the activation peptide and cleaves the remaining part into light and heavy chains. The activation process starts slowly because factor V itself has to be activated by the initial, small amounts of thrombin. Prothrombin / Coagulation Factor II is expressed by the liver and secreted in plasma. Defects in prothrombin / Coagulation Factor II are the cause of factor II deficiency (FA2D). It is very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Defects in Coagulation Factor II are also a cause of susceptibility to thrombosis. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation.