Rat F1+2(Prothrombin Fragment 1+2) EIA Kit
UNIProt ID
NA
Target Species
Rat
Target Name
F1+2
Target Synonym
F1+2
Type
Sandwich
Sensitivity
28.13pg/mL
Detection Type
Colormetric
Detection Range
46.88~3000pg/mL
Latin name
Rattus norvegicus
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Rat F1+2(Prothrombin Fragment 1+2) EIA Kit
Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
Description
This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test principle
This EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat F1+2. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat F1+2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat F1+2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat F1+2. You can calculate the concentration of Rat F1+2 in the samples by comparing the OD of the samples to the standard curve.