Rat PGF2α(Prostaglandin F2 Alpha) EIA Kit
UNIProt ID
NA
Target Synonym
NA
Target Species
Rat
Target Name
PGF2α
Sensitivity
4.69pg/mL
Type
Competitive
Detection Type
Colormetric
Detection Range
7.81~500pg/mL
Latin name
Rattus norvegicus
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
Rat PGF2α(Prostaglandin F2 Alpha) EIA Kit
Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Description
The PGF2α(Prostaglandin F2 Alpha) EIA Kit is a α- or alpha protein sometimes glycoprotein present in blood.This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
Test principle
This EIA kit uses the Competitive-EIA principle. The micro EIA plate provided in this kit has been pre-coated with Rat PGF2α. During the reaction, Rat PGF2α in the sample or standard competes with a fixed amount of Rat PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat PGF2α. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.