8-epi-PGF2α(8-Epi-Prostaglandin F2 Alpha) EIA Kit
UNIProt ID
NA
Sensitivity
9.38pg/mL
Target Species
Universal
Type
Competitive
Detection Type
Colormetric
Target Name
8-epi-PGF2α
Detection Range
15.63~1000pg/mL
Tested Sample Types
Serum, Plasma, Cell supernatant
Product Name
8-epi-PGF2α(8-Epi-Prostaglandin F2 Alpha) EIA Kit
Properties
MeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
Target Synonym
15-F2t-IsoP, 8-iso-PGF2a, 8-isoprostane, 8-iso-PG, 8-iso Prostaglandin
Description
The 8-epi-PGF2α(8-Epi-Prostaglandin F2 Alpha) EIA Kit is a α- or alpha protein sometimes glycoprotein present in blood.This ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
Test principle
This EIA kit uses the Competitive-EIA principle. The micro EIA plate provided in this kit has been pre-coated with 8-epi-PGF2α. During the reaction, 8-epi-PGF2α in the sample or standard competes with a fixed amount of 8-epi-PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 8-epi-PGF2α. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 8-epi-PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.