The Prostaglandin F2 Alpha (PGF2a) ELISA Kit, 96 is a α- or alpha protein sometimes glycoprotein present in blood.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Prostaglandin F2 Alpha (PGF2a) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Prostaglandin F2 Alpha (PGF2a) and unlabeled Prostaglandin F2 Alpha (PGF2a) (Standards or samples) with the pre-coated antibody specific to Prostaglandin F2 Alpha (PGF2a). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Prostaglandin F2 Alpha (PGF2a) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Prostaglandin F2 Alpha (PGF2a) in the sample.