Rat Prothrombin Fragment 1+2 (F1+2) CLIA Kit, 96 well plate

Rat Prothrombin Fragment 1+2 (F1+2) CLIA Kit, 96 well plate

Size

96T

Catalog no.

EKU09501

Price

1092 EUR

Buy

Verified applications

CLIA

Protein number

P18292

Research main area

Biology

Assay class

Sandwich

Shipping requirements

Blue ice

Use for

8 months

Sensitivity limit

0,23 ng/mL

Assay duration

2 hours, 40min

Detection limits

6.25-400 ng/mL

ELISA detection

Chemiluminescent

Latin name

Rattus norvegicus

Estimated production time

7-11 business days

Alternate gene name

Please refer to NCBI

Gene number

Please refer to GenBank

Verified reactivity

Rattus norvegicus (Rat)

Gene name

Prothrombin Fragment 1+2

Alternate protein number

Please refer to SwissProt

Protocol

Please see ELISA's datasheet, otherwise contact us

Notes

For research use only. Not for diagnostic procedures.

QC

The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Samples to be analyzed

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

ELISA's cross-reactivity

This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.

Warnings

Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage recommendation

-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

ELISA's specificity

This assay has high sensitivity and excellent specificity for detection of Prothrombin Fragment 1+2 (F1+2). No significant cross-reactivity or interference between Prothrombin Fragment 1+2 (F1+2) and analogues was observed.

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Precision of the test

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prothrombin Fragment 1+2 (F1+2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prothrombin Fragment 1+2 (F1+2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV

ELISA's stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay principle

The microplate provided in this kit has been pre-coated with an antibody specific to Prothrombin Fragment 1+2 (F1+2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Prothrombin Fragment 1+2 (F1+2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Prothrombin Fragment 1+2 (F1+2) level in the sample or standard.;

Description

A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.