Human Prothrombin Fragment 1+2 (F1+2) CLIA Kit, 96 well plate
Verified applications
CLIA
Gene number
2147
Protein number
P00734
Research main area
Biology
Shipping requirements
Blue ice
Assay class
Sandwich
Use for
8 months
Sensitivity limit
1,03 pg/mL
Assay duration
2 hours, 40min
ELISA detection
Chemiluminescent
Detection limits
31.25-2000 pg/mL
Estimated production time
7-11 business days
Alternate gene name
Please refer to NCBI
Verified reactivity
Homo sapiens (Human)
Gene name
Prothrombin Fragment 1+2
Alternate protein number
Please refer to SwissProt
Protocol
Please see ELISA's datasheet, otherwise contact us
Notes
For research use only. Not for diagnostic procedures.
QC
The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Samples to be analyzed
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
ELISA's cross-reactivity
This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Warnings
Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Storage recommendation
-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
ELISA's specificity
This assay has high sensitivity and excellent specificity for detection of Prothrombin Fragment 1+2 (F1+2). No significant cross-reactivity or interference between Prothrombin Fragment 1+2 (F1+2) and analogues was observed.
Precision of the test
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prothrombin Fragment 1+2 (F1+2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prothrombin Fragment 1+2 (F1+2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA's stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay principle
The microplate provided in this kit has been pre-coated with an antibody specific to Prothrombin Fragment 1+2 (F1+2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Prothrombin Fragment 1+2 (F1+2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Prothrombin Fragment 1+2 (F1+2) level in the sample or standard.;
Description
A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.