Trxf1/2, Thioredoxin F1/F2, chloroplastic
Notes
None
Raised in
Rabbit
Clonality
Polyclonal
Clone
Polyclonal
Molecular weight (expected | аpparent)
19.9 | 12 kDa
Protein number
Q9XFH9 , Q9XFH8
Verified applications
western blot (WB)
TAIR number
AT3G02730,AT5G16400
Verified reactivity
Arabidopsis thaliana
Purification
Affinity purified serum
Available ordering format
Lyophilized in PBS pH 7.4
Recommended dilutions for use
1 : 1000 with standard ECL (WB)
How to reconstitute
For reconstitution add 50 µl of sterile water.
References
to be added when available, antibody released in June 2015
No reactivity
no confirmed exceptions from predicted reactivity are currently known
Connected products
collection of antibodies involced in photosynthesisPlant and algal protein extraction buffer Secondary antibodies
Immunogen
KLH-conjugated peptide, derived from Arabidopsis thaliana Trxf1 UniProt: Q9XFH9, TAIR: AT5G16400 and Trxf2 UniProt: Q9XFH8, TAIR: AT3G02730
Supplementary information
5 mM DTT in extraction buffer and 5% B-ME in Lämmli buffer are recommended to use. Samples should be heated at 95°C for 2 min before loading as TRXs proteins have a tendency to oligomerize.
Scientific context
Trxf1/2 (Thioredoxin F1/F2, chloroplastic) involved in the redox regulation of enzymes of Calvin-Benson cycle and oxidative pentose phosphate pathway. Alternative names: Thioredoxin F1, F2 AtTrxf1, AtTrxf2
Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Possible reactivity
Aegilops tauschii, Brassica napus, Chlamydmonas reinhardtii, Fragaria ananassa, Glycine max, Glycine soja, Hyacinthus orientalis, Medicago truncatula, Mesembryanthemum crystallinum, Morus notabilis, Nicotiana tabacum, Oryza sativa, Pisum sativum, Populus trichocarpa, Ricinus communis, Spinacia oleracea, Theobroma cacao, Triticum urartu, Zea mays