General PGF2a(Prostaglandin F2 Alpha) ELISA Kit
Assay length
2h
Standard
800pg/mL
Sensitivity
3.88pg/mL
Detection range
9.88-800pg/mL
Alternative Names
PGF2A; PGF2-A; PG-F2a
Assay Type
Competitive Inhibition
Research Area
Metabolic pathway;Cardiovascular biology;Hormone metabolism;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Description
The General PGF2a(Prostaglandin F2 Alpha) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to PGF2a has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled PGF2a analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of PGF2a in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of PGF2a in the sample.