General 8-epi-PGF2a(8-Epi Prostaglandin F2 Alpha) ELISA Kit
Assay length
2h
Standard
2000pg/mL
Sensitivity
9.55pg/mL
Detection range
24.69-2000pg/mL
Assay Type
Competitive Inhibition
Alternative Names
8-EpiPGF2-α; 8epi-PGF2a
Research Area
Endocrinology;Pulmonology;Hepatology;Hormone metabolism;
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Description
The General 8-epi-PGF2a(8-Epi Prostaglandin F2 Alpha) ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) and unlabeled 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) (Standards or samples) with the pre-coated antibody specific to 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 8-Epi Prostaglandin F2 Alpha (8-epi-PGF2a) in the sample.