Mouse Monoclonal (IgG2a) to Human F2 / Prothrombin / Thrombin Antibody
Gene name
F2
Gene name synonims
N/A
Clone
N/A
Immunoglobulin isotype
IgG2a
French translation
anticorps
Category
Antibodies
Clonality
Monoclonal
Latin name
Mus musculus
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Concentration
1 miligram per 1 mililiter
Also known as
F2 / Prothrombin / Thrombin
Purification method
Ion exchange Chromatography
Other gene names
F2; F2; PT; THPH1; RPRGL2; N/A
Form/Appearance
Supplied as a liquid in 50% glycerol, ddH2O.
Tested applications:
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Storage and shipping
For short term storage keep the antibody at +4 degrees Celsius. For longer storage the antibody should be kept at -20 degrees Celsius. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies and reduce their activity.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Other names
prothrombin preproprotein; Prothrombin; prothrombin; serine protease; prothrombin B-chain; prepro-coagulation factor II; coagulation factor II (thrombin); Coagulation factor IICleaved into the following 4 chains:Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain
Specificity and cross-reactivity
Recognizes human Prothrombin, prethrombin-1, fragment 2, meizothrombin. Inhibits clotting and prothrombin activation.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.