Human
[TNFa]
Antibody
anticorps
Mus musculus
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
MyBioSource supplies antibodies that are for research of human proteins.Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. MyBioSource supplies azide free antibodies on request.Recently MyBioSource made this Mouse Anti- TNFa Capture Antibody, Clone B-F7[TNF-a ] available without azide. It will stick less and is more suitable for ELISA or in vivo studies. However you need to store the @VV@ frozen and ship on dry ice.