Antithrombin III Antibody, FITC Conjugated
Gene ID number
462
Conjugation
FITC
Modification site
None
Swiss Prot
P01008
Tested applications
IF(IHC-P)
French translation
anticorps
Modification
Unmodified
Clonality
Polyclonal
Immunogen_range
140-190/464
Excitation Emission
494nm/518nm
Concentration
1ug per 1ul
Target Antigen
Antithrombin III
Crossreactivity
Human, Mouse, Rat
Additional conjugation
Anti-FITC Antibody
Recommended dilutions
IF(IHC-P)(1:50-200)
Clone
Polyclonal antibody
Subcellular location
Extracellular, Secreted
Category
Conjugated Primary Antibodies
Host Organism
Rabbit (Oryctolagus cuniculus)
Purification method
This antibody was purified via Protein A.
Long name
Antithrombin III Polyclonal Antibody, FITC Conjugated
Specificity
This is a highly specific antibody against Antithrombin III.
Source
KLH conjugated synthetic peptide derived from human Antithrombin III
Synonyms
AT3; AT3D; ATIII; THPH7; Antithrombin-III; Serpin C1; SERPINC1; PRO0309
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Antigen background
Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.