Thrombin Activation peptide fragment 1 Antibody, Cy3 Conjugated
Conjugation
Cy3
Modification site
None
Gene ID number
2147
French translation
anticorps
Tested applications
IF(IHC-P)
Modification
Unmodified
Clonality
Polyclonal
Concentration
1ug per 1ul
Crossreactivity
Human, Mouse, Rat
Excitation Emission
512,550nm/570,615nm
Recommended dilutions
IF(IHC-P)(1:50-200)
Additional conjugation
cy3 conjugation kit
Clone
Polyclonal antibody
Category
Conjugated Primary Antibodies
Host Organism
Rabbit (Oryctolagus cuniculus)
Target Antigen
Thrombin Activation peptide fragment 1
Purification method
This antibody was purified via Protein A.
Long name
Thrombin Activation peptide fragment 1 Antibody, Cy3 Conjugated
Specificity
This is a highly specific antibody against Thrombin Activation peptide fragment 1.
Source
KLH conjugated synthetic peptide derived from human Thrombin Activation peptide fragment 1
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Synonyms
Activation peptide fragment 1; coagulation factor II; prothrombin; F2; Cf-2; Cf2; FII; F 2; coagulation factor II thrombin; Coagulation factor II; Coagulation factor II precursor; F2; Factor II; Factor-II; Prothrombin; prothrombin B-chain; PT; serine protease; THRB; THRB_HUMAN; Thrombin.
Additional isotype
Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
Description
Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-Thrombin Activation peptide fragment 1 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
Antigen background
Thrombin is the final protease in the blood coagulation cascade and serves both pro- and anticoagulant functions through the cleavage of several targets. The ability of thrombin to specifically recognize a wide range of substrates derives from interactions which occur outside of the active site of thrombin. Thrombin possesses two anion binding exosites which mediate many of its interactions with cofactors and substrates, and although many structures of thrombin have been solved, few such interactions have been described in molecular detail. Glycosaminoglycan binding to exosite II of thrombin plays a major role in switching off the procoagulant functions of thrombin by mediating its irreversible inhibition by circulating serpins and by its binding to the endothelial cell surface receptor thrombomodulin.