Thrombin receptor Antibody, FITC Conjugated
Gene ID number
2149
Conjugation
FITC
Modification site
None
Swiss Prot
P25116
Tested applications
IF(IHC-P)
French translation
anticorps
Modification
Unmodified
Clonality
Polyclonal
Immunogen_range
290-315/425
Excitation Emission
494nm/518nm
Concentration
1ug per 1ul
Target Antigen
Thrombin receptor
Crossreactivity
Human, Mouse, Rat
Additional conjugation
Anti-FITC Antibody
Subcellular location
Cytoplasm, Helical
Recommended dilutions
IF(IHC-P)(1:50-200)
Clone
Polyclonal antibody
Category
Conjugated Primary Antibodies
Host Organism
Rabbit (Oryctolagus cuniculus)
Purification method
This antibody was purified via Protein A.
Long name
Thrombin receptor Polyclonal Antibody, FITC Conjugated
Source
KLH conjugated synthetic peptide derived from human PAR1
Specificity
This is a highly specific antibody against Thrombin receptor.
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Synonyms
TR; HTR; CF2R; PAR1; PAR-1; Proteinase-activated receptor 1; Coagulation factor II receptor; Thrombin receptor; F2R
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Antigen background
High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.
Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.