Sheep anti-human Thrombin (FIIa), FITC-Conjugated Affinity-Purified IgG

Sheep anti-human Thrombin (FIIa), FITC-Conjugated Affinity-Purified IgG

Size

100ug

Catalog no.

MBS512152

Price

453 EUR

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Additional isotype

IgG

Gene name

N/A

Gene name synonims

N/A

Other gene names

N/A

Immunoglobulin isotype

N/A

Tested for:

N/A

Species reactivity

N/A

Host organism

Sheep

Alternative name4

Thrombin

Clonality

Polyclonal

Category

Antibodies

Other names

thrombin; N/A

Alternative name5

Thrombin, human

Conjugation

Anti-FITC Antibody

Clone

Polyclonal antibody

Form/Appearance

APIgG-FITC conjugate as a clear yellow liquid.

Storage and shipping

Store at 2 degree C to 8 degree C and protect from light.

Alternative name2

Ovine anti-human Thrombin (FIIa), FITC-Conjugated Affinity-Purified IgG

Alternative name1

Anti-Sheep anti-human Thrombin (FIIa), FITC-Conjugated Affinity-Purified IgG

Alternative name3

Ovinae anti-Homo sapiens Thrombin (FIIa), FITC-Conjugated Affinity-Purified IgG

Concentration

APIgG-FITC concentration is mg/mL, determined by absorbance using an extinction coefficient (E1%280) of 14.

Specificity and cross-reactivity

Prior to conjugation, this antibody was specific for thrombin (including prothrombin) as demonstrated by immunoelectrophoresis and ELISA.

Purification method

Affinity purified, FITC conjugated IgG. Vial containing mL of affinity-purified IgG conjugated to fluorescein isothiocyanate (FITC). Total protein is 0.1 mg.

Additional description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Test

A high affinity purification column was use to purify anti- Thrombin (FIIa) FITC-Conjugated -Purified IgG by MBS Polyclonals by chromatographic size exclusion.MBS Polyclonals supplies antibodies that are for research of human proteins.

Advisory

In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Description

productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.

Properties

This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.