Prothrombin IgG/IgM ELISA kit
Tested for
ELISA
Additional isotype
IgG,IgM
Shipping conditions
Blue Ice
Area of research
Immunology
Category
Research kit
Storage
Store at 2-8 deg C
Usage Recommendations
Optimal conditions to be determined by end-user
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
Assay Information
The AntiProthrombin IgG/IgM is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of IgG or IgM class autoantibodies directed against Prothrombin. The microplate is coated with highly purified prothrombin. The microplate can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. Each well can be separated from the module ("breakaway"). The binding of present autoantibodies, formation of the sandwich complexes and enzymatic colour reaction take place during three different reaction phases. To read the optical density a microplate reader with a 450 nm filter is required. Bichromatic measurement with a 600690 nm reference is recommended.