Prothrombin IgG/IgM ELISA kit

Prothrombin IgG/IgM ELISA kit

Size

96 wells

Catalog no.

55R-ORG541

Price

449 EUR

Buy

Tested for

ELISA

Additional isotype

IgG,IgM

Shipping conditions

Blue Ice

Area of research

Immunology

Category

Research kit

Storage

Store at 2-8 deg C

Usage Recommendations

Optimal conditions to be determined by end-user

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen

About

Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.

Assay Information

The AntiProthrombin IgG/IgM is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of IgG or IgM class autoantibodies directed against Prothrombin. The microplate is coated with highly purified prothrombin. The microplate can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. Each well can be separated from the module ("breakaway"). The binding of present autoantibodies, formation of the sandwich complexes and enzymatic colour reaction take place during three different reaction phases. To read the optical density a microplate reader with a 450 nm filter is required. Bichromatic measurement with a 600690 nm reference is recommended.