Thrombin R (G17) polyclonal antibody
Applications
WB
Swiss-Prot
P25116
BiowMW
~ 47 kDa
French translation
anticorps
Reactivity
Human,Mouse,Rat
Application_all
WB: 1:500~1:1000
Group
Polyclonals and antibodies
Note
For research use only, not for use in diagnostic procedure.
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Immunogen
Synthetic peptide, corresponding to the N-terminal of Human Thrombin R.
Specificity
Thrombin R (G17) polyclonal antibody detects endogenous levels of Thrombin R protein
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name
Proteinase-activated receptor 1; PAR-1; Coagulation factor II receptor; Thrombin receptor; F2R; CF2R; PAR1; TR
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Purification&Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Usage
Western blot (WB) analysis of Thrombin R (G17) pAb at 1:500 dilution Lane1:PC3 whole cell lysate(40ug) Lane2:U-87MG whole cell lysate(40ug) Lane3:The Brian tissue lysate of Rat(10ug) Lane4:The Brain tissue lysate of Mouse(20ug)
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.