Prothrombin polyclonal antibody
Applications
WB
Swiss-Prot
P00734
BiowMW
~ 75 kDa
French translation
anticorps
Reactivity
Human,Mouse,Rat
Application_all
WB: 1:500~1:1000
Group
Polyclonals and antibodies
Note
For research use only, not for use in diagnostic procedure.
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Immunogen
Synthetic peptide, corresponding to amino acids430-480 of Human Prothrombin.
Specificity
Prothrombin polyclonal antibody detects endogenous levels of Prothrombin protein.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Alternative Name
Prothrombin; Coagulation factor II; Activation peptide fragment 1; Activation peptide fragment 2; Thrombin light chain; Thrombin heavy chain; F2
Purification&Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Usage
Western blot (WB) analysis of Prothrombin polyclonal antibody at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:L02 whole cell lysate(40ug) Lane4:The Liver tissue lysate of Rat(40ug) Lane5:The Liver tissue lysate of Mouse(40ug)