Thrombin R (S42) polyclonal antibody
Applications
WB
Swiss-Prot
P25116
BiowMW
~ 48 kDa
French translation
anticorps
Reactivity
Human,Mouse,Rat
Application_all
WB: 1:500~1:1000
Group
Polyclonals and antibodies
Note
For research use only, not for use in diagnostic procedure.
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Immunogen
Synthetic peptide, corresponding to amino acids 12-60 of Human Thrombin R.
Specificity
Thrombin R (S42) polyclonal antibody detects endogenous levels of Thrombin R protein.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alternative Name
Proteinase-activated receptor 1; PAR-1; Coagulation factor II receptor; Thrombin receptor; F2R; CF2R; PAR1; TR
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Usage
Western blot (WB) analysis of Thrombin R (S42) pAb at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:AML-12 whole cell lysate(40ug)
Purification&Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.