Human Serpin C1/Antithrombin-III PicoKine ELISA Kit
Entrez nr.
462
Minutes of TMB incubation
25-30
Gen protein identity
P01008
ELISA's sensitivity
< 10pg/ml
Antigen
NSO, H33-K464
Gene tested
human Serpin C1
Uniprot
Antithrombin-III
ELISA kit detection limits
312pg/ml to 20000pg/ml
Anti-coagulation
heparin, EDTA or citrate
Target of this ELISA
Natural and recombinant human Serpin C1
Gene name
serpin peptidase inhibitor, clade C (antithrombin), member 1
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
ELISA's application
For quantitative measurement of human Serpin C1 in cell culture supernatant, serum, plasma heparin, EDTA, citrate and urine.
ELISA standard curve
Concentration(pg/ml) + 0 + 312 + 625 + 1250 + 2500 + 5000 + 10000 + 20000 O.D. + 0.011 + 0.062 + 0.082 + 0.15 + 0.295 + 0.582 + 1.333 + 1.974
ELISA's precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);1.05;3.42;8.67;1.32;3.74;8.23 ELISA standard deviations 0.064;0.194;0.546;0.088;0.239;0.543 CV(%);6.1;5.7;6.3;6.7;6.4;6.6
Alternative
ANT3_HUMAN;Antithrombin;Antithrombin III;Antithrombin-III;AntithrombinIII;AT 3;AT III;AT3;AT3D;ATIII;Heparin cofactor I;MGC22579; Serine (or cysteine) proteinase inhibitor clade C (antithrombin) member 1;Serine cysteine proteinase inhibitor clade C member 1; Serine proteinase inhibitor clade C member 1;Serpin C1;Serpin peptidase inhibitor clade C (antithrombin) member 1;SERPINC1;THPH7
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human Serpin C1 antibodies;1 ST0000-20;Lyophilized recombinant human Serpin C1 standard;20ng/tube×2 AR1107;Biotinylated anti- human Serpin C1 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA kit description
GENTAUR's human Serpin C1 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for Serpin C1 has been precoated onto 96-well plates. Standards (NSO, H33-K464) and test samples are added to the wells; a biotinylated measurement polyclonal antibodies from goat specific for Serpin C1 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human Serpin C1 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
How the elisa works
Sample the amount of 100ul/well of the standard solution at 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human Serpin C1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum, plasma (heparin, EDTA, citrate) or urine to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each human Serpin C1 standard solution and each sample is measured in duplicate.
More gene info
Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity directly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22% of the intron sequence) than in the genome as a whole (about 5%).