Thrombin heavy chain Antibody, ALEXA FLUOR 488
Gene ID Number
2147
Modification Site
None
Tested applications
IF(IHC-P)
French translation
anticorps
Modification
Unmodified
Clonality
Polyclonal
Excitation emission
499nm/519nm
Conjugation
Alexa Fluor
Concentration
1ug per 1ul
Crossreactivity
Human, Mouse, Rat
Conjugated with
ALEXA FLUOR® 488
Recommended dilutions
IF(IHC-P)(1:50-200)
Clone
Polyclonal antibody
Target Antigen
Thrombin heavy chain
Purification
Purified by Protein A.
Category
Conjugated Primary Antibodies
Host Organism
Rabbit (Oryctolagus cuniculus)
Also known as
Anti-Thrombin heavy chain PAb ALEXA FLUOR 488
Specificity
This is a highly specific antibody against Thrombin heavy chain.
Long name
Thrombin heavy chain Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
Cross-reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Thrombin heavy chain
Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Synonyms
coagulation factor II; prothrombin; F2; Cf-2; Cf2; FII; F 2; coagulation factor II thrombin; Coagulation factor II; Coagulation factor II precursor; F2; Factor II; Factor-II; Prothrombin; prothrombin B-chain; PT; serine protease; THRB; THRB_HUMAN; Thrombin; Thrombin heavy chain.
Properties
For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Thrombin heavy chain has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Background of the antigen
Thrombin is the final protease in the blood coagulation cascade and serves both pro- and anticoagulant functions through the cleavage of several targets. The ability of thrombin to specifically recognize a wide range of substrates derives from interactions which occur outside of the active site of thrombin. Thrombin possesses two anion binding exosites which mediate many of its interactions with cofactors and substrates, and although many structures of thrombin have been solved, few such interactions have been described in molecular detail. Glycosaminoglycan binding to exosite II of thrombin plays a major role in switching off the procoagulant functions of thrombin by mediating its irreversible inhibition by circulating serpins and by its binding to the endothelial cell surface receptor thrombomodulin.