Thrombin Activation peptide fragment 1 Antibody, ALEXA FLUOR 488
Modification Site
None
Gene ID Number
2147
French translation
anticorps
Tested applications
IF(IHC-P)
Modification
Unmodified
Clonality
Polyclonal
Excitation emission
499nm/519nm
Concentration
1ug per 1ul
Conjugation
Alexa Fluor
Conjugated with
ALEXA FLUOR® 488
Crossreactivity
Human, Mouse, Rat
Clone
Polyclonal antibody
Recommended dilutions
IF(IHC-P)(1:50-200)
Purification
Purified by Protein A.
Category
Conjugated Primary Antibodies
Host Organism
Rabbit (Oryctolagus cuniculus)
Target Antigen
Thrombin Activation peptide fragment 1
Also known as
Anti-Thrombin Activation peptide fragment 1 PAb ALEXA FLUOR 488
Long name
Thrombin Activation peptide fragment 1 Antibody, ALEXA FLUOR 488 Conjugated
Specificity
This is a highly specific antibody against Thrombin Activation peptide fragment 1.
Cross-reactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Source
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Thrombin Activation peptide fragment 1
Storage conditions
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Synonyms
Activation peptide fragment 1; coagulation factor II; prothrombin; F2; Cf-2; Cf2; FII; F 2; coagulation factor II thrombin; Coagulation factor II; Coagulation factor II precursor; F2; Factor II; Factor-II; Prothrombin; prothrombin B-chain; PT; serine protease; THRB; THRB_HUMAN; Thrombin.
Additional isotype
Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
Description
Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
Properties
For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Thrombin Activation peptide fragment 1 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Background of the antigen
Thrombin is the final protease in the blood coagulation cascade and serves both pro- and anticoagulant functions through the cleavage of several targets. The ability of thrombin to specifically recognize a wide range of substrates derives from interactions which occur outside of the active site of thrombin. Thrombin possesses two anion binding exosites which mediate many of its interactions with cofactors and substrates, and although many structures of thrombin have been solved, few such interactions have been described in molecular detail. Glycosaminoglycan binding to exosite II of thrombin plays a major role in switching off the procoagulant functions of thrombin by mediating its irreversible inhibition by circulating serpins and by its binding to the endothelial cell surface receptor thrombomodulin.