anti-Prothrombin Antibody
Target of this antibody:
F2
This antibody has been succesfully tested for use in following methods:
WB
Clone:
N/A
This antibody reacts with:
Human
Antibody host:
Rabbit
Form of antibody:
Liquid
Shipping conditions:
gel pack
Concentration of antibody:
0.5 mg/ml
French translation
anticorps
Isotype:
Rabbit IgG
Clonality of antibody:
Polyclonal
Recommended usage:
WB: 1-4 µg
Alternative names of antibody target:
Coagulation factor II
Purification method:
Affinity purified rabbit IgG
Immunogene sequence:
Human Prothrombin (Cat. No. 7684-1)
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
Formulation of this antibody:
In PBS pH 7.2, 0.01 % BSA, 0.03 % ProClin® and 50 % glycerol
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Background information:
Prothrombin is a vitamin K-dependent plasma protein which is synthesized in the liver. Prior to secretion into plasma, prothrombin undergoes post-translational modification by a vitamin K-dependent carboxylase which converts ten specific glutamic acid residues to γ-carboxyglutamic acid (gla). Conversion to thrombin is a key step in the blood coagulation pathway and catalyzes the coagulation of fibrinogen. Clinically, cases of selective deficiency are rare, although, in cases of liver cirrhosis, prothrombin is decreased. During activation, prothrombin is cleaved at Arg271-Thr272 and at Arg320-Ser321 to a "pro" fragment (fragment 1.2) and thrombin, the latter of which is composed of two chains covalently linked by a disulfide bond. There is an additional thrombin feed-back cleavage at Arg284-Thr285 resulting in an additional 13 amino acids being removed from the mature thrombin “A” chain.