Thrombin Cleavage Kit
Kit's other name
none
Storage condition
-20°C
Shipping condition
gel pack
Maximum time can be stored
12 months
Highlights
• SDS:PAGE
Species reactivity
see datasheet
Category
Thrombin, Thrombin cleavage, Tag removal by Thrombin
Samples tested
Fusion or affinity-tagged protein containing Thrombin-specific cleavage site.
Contents
• Thrombin Dilution Buffer • Thrombin Cleavage Buffer • Thrombin Enzyme • Cleavage Control Protein
Applications
• Efficiently removes tags from recombinant fusion proteins containing accessible Thrombin-specific recognition sequence.
Kit's benefits
• Simple procedure, fast and efficient cleavage of tags • The kit contains an active Thrombin enzyme sufficient to cleave up to 5 mg of target protein. • A 6x His-tagged protein containing the Thrombin cleavage site is included as a control. • Following cleavage of the target protein, Thrombin can be conveniently removed using a Heparin Sepharose column.
Kit's description
Thrombin enzyme (Factor IIa, EC 3.4.21.5) is an important clotting factor that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. It is also used as a valuable biochemical tool due to its high proteolytic specificity. Thrombin cleavage site (e.g. Leu-Val-Pro-Arg-ll-Gly-Ser; ll: cleavage site) is widely incorporated within the linker region of fusion or affinity tagged recombinant proteins. After successful cleavage with Thrombin, affinity tags or fused proteins can be separated from the target protein. Biovision's Thrombin Cleavage Kit provides an easy approach to test and optimize cleavage conditions of a target fusion or affinity-tagged protein containing Thrombin-specific cleavage site. The kit contains an active Thrombin enzyme sufficient to cleave up to 5 mg of the target protein. A 6xHis-tagged protein containing the Thrombin cleavage site is included as a Cleavage Control Protein. Following cleavage of the target protein, Thrombin can be removed by passing the reaction mix through the Heparin Sepharose column (Catalog # 6553 and 6554).