Prothrombin Antibody (Thrombin)
Tested applications
WB
Uniprot #
P18292
Category
Antibody
French translation
anticorps
Conjugation
Unconjugated
Purity
Antigen affinity
Clone
Polyclonal antibody
Recognised antigen
Prothrombin (Thrombin)
Recommended dilutions
Western blot: 0.5-1ug/ml
Form
Antigen affinity purified
Clonality
Polyclonal (rabbit origin)
Host animal
Rabbit (Oryctolagus cuniculus)
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Intented use
This Prothrombin antibodyis to be used only for research purposes and not for diagnostics..
Immunogen
Amino acids 600-612 (THVFRLKRWMQKV-rat) were used as the immunogen for this Prothrombin antibody.
Notes
Titration of the Prothrombin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Species reactivity
Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Storage
After reconstitution, the Prothrombin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Description
F2 (Coagulation Factor II), also known as prothrombin and thrombin, is a serine protease that in humans is encoded by the F2 gene. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII(F8), and XIII(F13A1). Thrombin also inhibits coagulation by Activating protein C.