Antithrombin III Antibody
Tested applications
WB
Uniprot #
P01008
Category
Antibody
French translation
anticorps
Conjugation
Unconjugated
Purity
Antigen affinity
Recognised antigen
Antithrombin III
Clone
Polyclonal antibody
Recommended dilutions
Western blot: 0.5-1ug/ml
Form
Antigen affinity purified
Clonality
Polyclonal (rabbit origin)
Host animal
Rabbit (Oryctolagus cuniculus)
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Intented use
This Antithrombin III antibodyis to be used only for research purposes and not for diagnostics..
Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Immunogen
An amino acid sequence from the N-terminus of human AT3 (TFYQHLADSKNDNDN) was used as the immunogen for this Antithrombin III antibody.
Notes
The stated application concentrations are suggested starting amounts. Titration of the Antithrombin III antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Storage
After reconstitution, the Antithrombin III antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Description
Antithrombin III (AT3), is the most important inhibitor of thrombin and other coagulation proteinases. It is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency.